Mechanism of Chloramphenicol Resistance in Staphylococcus epidermidis

Mechanism of Chloramphenicol Resistance in Staphylococcus epidermidis.

The mechanism of chloramphenicol resistance in several multiple-resistant Staphylococcus epidermidis strains has been studied and shown to be due to the presence of the enzyme, chloramphenicol acetyltransferase. As with S. aureus, the inactivating enzyme in S. epidermidis appears to be the product of a structural gene on the chloramphenicol plasmid because resistance and enzyme activity are con...

Transduction of tetracycline resistance in Staphylococcus epidermidis.

Fourteen Staphylococcus epidermidis strains were biotyped according to the scheme of Baird-Parker and tested for their sensitivities to S. epidermidis typing phages. Tetracycline-susceptible strains were tested for the capacity to be transduced to resistance. Five strains, all of biotype 1, were lysed by one or more phages. Five of eight biotype 1 strains were transduced to tetracycline resista...

Plasmid for tetracycline resistance in Staphylococcus epidermidis.

A plasmid determining tetracycline resistance was demonstrated for Staphylococcus epidermidis. Tetracycline resistance was spontaneously lost from a S. epidermidis strain at a frequency of <0.1 to 1.3%. Frequency of loss was not appreciably enhanced by growth at 42 C or in ethyl violet. The rate of transduction of tetracycline resistance in three S. epidermidis crosses was decreased as a functi...

Mechanism of R factor-mediated chloramphenicol resistance.

Transfer of resistance plasmids from Staphylococcus epidermidis to Staphylococcus aureus: evidence for conjugative exchange of resistance.

The ability of Staphylococcus epidermidis to transfer antimicrobial resistance to Staphylococcus aureus was tested by mixed culture on filter membranes. Two of six clinical isolates examined were able to transfer resistance to S. aureus strains 879R4RF, RN450RF, and UM1385RF. Subsequent S.aureus transconjugants resulting from matings with S. epidermidis donors were able to serve as donors to ot...